Chronotype refers to individual differences in the timing of circadian sleep-wake cycles and subjective alertness throughout the day. It is a potential factor influencing people's driving behaviour, but no research has explored the mechanisms underlying this topic. The current study aimed to explore the relationship between morningnesseveningness preferences and driving behaviour and the mediating effect of visual search between them. Thirty-eight drivers were selected to participate in this study based on their chronotype. They were divided into morning-type and evening-type groups by their score on the reduced Morningness-Eveningness Questionnaire (rMEQ). Both groups completed a visual search task and a simulated driving task in a morning session and an evening session. The results showed that morningness-eveningness preferences had synchronous effects on basic driving performance; specifically, morning-type drivers showed better driving behaviour in the morning than in the evening, and evening-type drivers showed the opposite trend. Furthermore, visual search abilities showed a mediating effect between morningness-eveningness preference and driving performance. The mechanisms underlying these results are discussed. Related results and data could aid with schedule arrangements for professional drivers.